External Affairs Minister S M Krishna urged the Sri Lankan government to release the fishermen, who were also accused of ramming into Lankan naval ships, on humanitarian basis immediately, official sources said.
Fisheries department officials in Rameswaram had said in Tamil Nadu last week that Sri Lankan Naval personnel allegedly attacked some fishing boats and arrested 23 Tamil Nadu fishermen when they were fishing near Katchatheevu in the Palk Straits.
Rameswaram Fishermen that the Lankan naval men stated tacked 20 other fishing boats and seized the fishing nets.
Katchatheevu, an islet, was ceded to Sri Lanka by India in 1974.
Earlier on July one, ten fishermen from Tamil Nadu were taken into custody by the Lankan Navy for reportedly crossing the International Maritime Boundary Line and fishing in their waters but were subsequently ordered to be released by a Mannar court.
Following their arrest, Tamil Nadu Chief Minister Jayalalithaa had shot off a letter to Prime Minister Manmohan Singh, expressing concern over "unrelenting, harsh attitude of the Sri Lankan Navy" towards Indian fishermen.
She had also sought the Centre's intervention in securing the release of those arrested by the island republic's navy.